龙眼类受体蛋白激酶CRK家族全基因组鉴定及表达调控分析

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摘  要  为了解龙眼富含半胱氨酸的类受体蛋白激酶（cysteine-rich receptor-like kinase，CRK）家族的基本特征与功能，本研究采用生物信息学分析方法对筛选出的98个龙眼CRK基因家族的成员进行分析鉴定，包括分子进化树的构建和对蛋白结构域及其特性、启动子及其顺式作用元件、体胚发生的过程以及组织器官特异性表达的分析。结果显示：通过对龙眼和拟南芥CRK基因做进化树分析，可根据亲缘关系的远近将其分为7个亚家族;不同亚家族成员的蛋白质特性（氨基酸数量、相对分子质量、等电点、信号肽及糖基化位点）有所不同;各成员基因结构特性，启动子顺式作用元件，蛋白结构域特性等与进化树中家族成员亲缘关系的远近相关;98个CRK家族成员在体胚发生过程（NEC、EC、ICpEC、GE）以及生长发育过程中组织器官特异性表达情况有所不同，其中在非胚性愈伤组织以及幼果中表达量较高，在种子和果实中几乎未表达。此外，富含半胱氨酸的类受体蛋白激酶在生物胁迫、非生物胁迫（抗病虫害、抗旱、抗寒、抗盐胁迫等）以及在植物生长发育过程中起着重要作用。

关键词  龙眼;CRK;基因家族;鉴定;表達调控中图分类号  S667.2      文献标识码  A

Genome-wide Identification and Regulation of Expression Analysis of Cysteine-rich Receptor-like Kinase in Longan

SUN Ying， LIN Yiling， ZHAO Pengcheng， XU Xiaoping， CHEN Xiaohui， SHEN Xu， LIN Yuling，LAI Zhongxiong^*

Institute of Horticultural Biotechnology， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China

Abstract  In order to understand the basic characteristics and functions of cysteine-rich receptor-like kinase （CRK） family in longan， a series of bioinformatics methods were used to analyze and identify 98 members of CRK gene family members， including constructing the molecular evolutionary tree， analyzing protein domains and characteristics， promoters， homeopathic elements， somatic embryogenesis and tissue-organ specific expression. Results showed that the CRK gene of longan and Arabidopsis could be divided into seven subfamilies according to the distance of the relationship by evolutionary tree analysis， and the protein characteristics （the quantity of amino acids， the relative molecular weight， the isoelectric point， the signal peptide and sites of glycosylation） of different subfamilies were different. Structural characteristics of the genes of each member， promoter homeopathic elements and protein domain characteristics were closely related to family members in the evolutionary tree. The specific expression of 98 CRK family members during somatic embryogenesis （NEC， EC， ICpEC， GE） and growth were different， particularly higher in non-embryogenic callus and young fruits， but they were almost not expressed in seeds and matured fruits. In addition， cysteine-rich receptor-like protein kinases played important roles in biological stress， abiotic stress （insect-induce diseases， drought resistance， cold resistance， salt resistance， etc.） and it played important roles in plant growth and development as well.